Rotavirus Occurrence in Shellfish with Low Levels of E. coli

The purpose of this study was to evaluate “in field” the accumulation of virus in shellfish and compare it with the concentration of bacterial indicators. Individuals of Mytilus galloprovincialis were placed in two sampling station located in a contaminated coastal bay and in one control station located one kilometer offshore. The presence of Rotavirus and E. coli was assessed weekly both in seawater and in shellfish samples. The Rotavirus genome was detected in water, preliminarily concentrated by tangential flow ultrafiltration method, and in hepatopancreas of mussels by Real-Time PCR. E. coli was enumerated in water matrices by a filtering method and in mussels by the MPN method. Rotaviruses were not recorded in seawater, while in mussels they were detected since third week after placement. E. coli in mussels were always below the limits set in the Regulation (EC) 854/2004. This study suggests the need for a viral indicator to insure the safety for consumption of shellfish.     

The Mar Piccolo of Taranto: an interesting marine ecosystem for the environmental problems studies

The National Project RITMARE (la Ricerca ITaliana per il MARE—Italian Research for the sea) started from 1 January 2012. It is one of the national research programs funded by the Italian Ministry of University and Research. RITMARE is coordinated by the National Research Council (CNR) and involves an integrated effort of most of the scientific community working on marine and maritime issues. Within the project, different marine study areas of strategic importance for the Mediterranean have been identified: Among these, the coastal area of Taranto (Ionian Sea, Southern Italy) was chosen for its different industry settlements and the relative impact on the marine environment. In particular, the research has been concentrated on the Mar Piccolo of Taranto, a complex marine ecosystem model important in terms of ecological, social, and economic activities for the presence also of extensive mussel farms. The site has been selected also because the Mar Piccolo area is a characteristic “on field” laboratory suitable to investigate release and diffusion mechanisms of contaminants, evaluate chemical–ecological risks towards the marine ecosystem and human health, and suggest and test potential remediation strategies for contaminated sediments. In this context, within the project RITMARE, a task force of researchers has contributed to elaboration a functioning conceptual model with a multidisciplinary approach useful to identify anthropogenic forcings, its impacts, and solutions of environmental remediation. This paper describes in brief some of the environmental issues related to the Mar Piccolo basin.     

Potential environmental factors influencing mucilage formation in the northern Adriatic Sea

In the northern Adriatic Sea, Mediterranean Sea, the mucilage phenomenon is usually related to the aggregation of extracellular organic matter of phytoplanktonic origin under special seasonal and trophic conditions. Environmental factors, such as temperature, salinity, nitrogen, phosphorus and N/P ratio as well as polysaccharide and monosaccharide levels in the presence and absence of mucilage were investigated in the northern Adriatic Sea. Variations in temperature and salinity during spring in years characterised by mucilage events have been recognised having an important role in preparing a favourable physical environment where mucilage formation could take place. Since the DIN/TP ratio increased markedly during the spring months (March–April) in 2002, 2003 and 2004, preceding the early summer mucilage outbreaks, it appeared that the DIN/TP ratio may be a more robust predictor than the DIN/DIP ratio, which, although more commonly used, exhibited a discontinuous trend. The contents of total carbohydrates, including monosaccharides and polysaccharides, in seawater also varied according to the presence or absence of mucilage, with an increase of polysaccharide levels during the late spring or early summer in the period of the mucilage appearance. The role of carbohydrates in the macro-aggregate formation was also investigated.     

Using OpenMI and a Model MAP to Integrate WaterML2 and NetCDF Data Sources into Flood Modeling of Genoa,Italy

Extreme hydrometeorological events such as flash floods have caused considerable loss of life and damage to infrastructure over recent years. Flood events in the Mediterranean region between 1990 and 2006 caused over 4,500 fatalities and cost over €29 billion in damage, with Italy one of the worst affected countries. The Distributed Computing Infrastructure for Hydro‐Meteorology (DRIHM) project is a European initiative aiming at providing an open, fully integrated eScience environment for predicting, managing, and mitigating the risks related to such extreme weather phenomena. Incorporating both modeled and observational data sources, it enables seamless access to a set of computing resources with the objective of providing a collection of services for performing experiments with numerical models in meteorology, hydrology, and hydraulics. The purpose of this article is to demonstrate how this flexible modeling architecture has been constructed using a set of standards including the NetCDF and WaterML2 file formats, in‐memory coupling with OpenMI, controlled vocabularies such as CF Standard Names, ISO19139 metadata, and a Model MAP (Metadata, Adaptors, Portability) gateway concept for preparing numerical models for standardized use. Hydraulic results, including the impact to buildings and hazards to people, are given for the use cases of the severe and fatal flash floods, which occurred in Genoa, Italy in November 2011 and October 2014.     

Prenatal diagnosis and management of fetal ovarian cysts

Congenital ovarian cysts are a pathological condition which can be diagnosed in utero by ultrasound. We report 14 consecutive diagnoses of fetal ovarian cysts, obtained in the second and third trimesters of pregnancy. Congenital ovarian cysts have almost invariably a good prognosis, and no change in the standard obstetrical management is required. However, the evolution of the disease in utero is extremely variable. The cyst may increase in size, decrease or even disappear, or undergo complications such as torsion and rupture, which may carry some risks to the fetus. When a fetal ovarian cyst is detected, serial ultrasound examinations should be performed. If one of the above complications is suspected, the option of prompt caesarean section should be considered.     

First trimester prenatal diagnosis of Sanfilippo disease (MPSIII) type B

Two pregnancies of a family at risk for Sanfilippo disease type B were monitored in the first trimester. In one case an affected fetus was diagnosed on chorionic villi by the assay of N-acetyl-a-D -glucosaminidase and confirmed on cultured fibroblasts from the aborted fetus. Pathological findings are also reported and compared with changes observed later in life. The disease was excluded in the second pregnancy.     

Agriculture,livelihoods and climate change in the West African Sahel

The West African Sahel is a harsh environment stressed by a fast-growing population and increasing pressure on the scarce natural resources. Agriculture is the main source of livelihood of the majority of the people living in the area. Increases in temperature and/or modifications in rainfall quantities and distribution will substantially impact on the natural resource on which agriculture depends. The vulnerability of livelihoods based on agriculture is increased and most likely exacerbate and accelerate the current ‘downward spiral’ of underdevelopment, poverty and environmental degradation. Notably, droughts, a short rainy season and/or very low rainfall will be felt by current systems. To cope with the difficult climatic situation, farm households have developed a range of strategies including selling of animals and on-farm diversification or specialization. At regional level, early warning systems including an operational agro-meteorological information system already provide farmers with crucial information. Substantial political, institutional and financial efforts at national and international level are indispensable for the sustenance of millions of lives. In terms of development, priority needs to be given to adaptation and implementation of comprehensive programs on water management and irrigation, desertification control, development of alternative sources of energy and the promotion of sustainable agricultural practices by farmers.